In-Vivo Toxicity Assessment of Biologically Synthesized Iron Oxide Nanoparticles in Zebrafish (Danio Rerio)

Shazmin Mohiuddin Hafiz¹, Smital Sameer Kulkarni¹ and Mansee Kapil Thakur²

¹Department of Medical Biotechnology - MGM School of Biomedical Sciences, MGMIHS, Kamothe, Navi Mumbai, Maharashtra, India.

²Department of Medical Biotechnology and Central Research Laboratory, MGM School of Biomedical Sciences, MGMIHS and MGMCET, MGM Medical College building, MGM Institute of Health Sciences, Kamothe, Navi Mumbai 410209, Maharashtra, India.

Corresponding Author E-mail: mansibiotech79@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2645

ABSTRACT: Nanoparticles are increasingly used for biomedical purposes. In the past decades, much attention has been paid to toxicity assessment of nanoparticles prior to clinical and biological applications. Iron oxide nanoparticles (IONPs) are being introduced into the consumer market significantly. Effects and accumulation patterns of IONPs were studied using zebrafish embryos. Nanoparticles of iron were synthesized by biological reduction of spinach, using 0.1 M Ferric Chloride. These nanoparticles were characterized by SEM-EDS, XRD, FTIR and AAS were found to have the size range of 100 to 250 nm. Fish (n=12/group) were exposed from 8hpf to 7dpf to 1, 5, 10, 50 and 100 mg/L IONPs. The results showed that LC₅₀ was found to be10 mg/L concentration of iron oxide nanoparticles. 50 mg/L and 100 mg/L concentrations showed developmental toxicity in these embryos, causing mortality, and hatching delay. This study is one of the first on developmental toxicity in zebra fish caused by using biologically synthesised iron oxide nanoparticles.

KEYWORDS: FTIR; Iron Oxide Nanoparticles; SEM; Toxicity XRD; Zebrafish;

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