Antibacterial Activity and Morphological Characterization of Synthesis Graphene Oxide Nanosheets by Simplified Hummer's Method

Russel R. Ghanim, M. R. Mohammad and Adi M. Abdul Hussien

Branch of Applied Physics, Department of Applied Science, University of Technology, Baghdad-Iraq.

Corresponding Author E-mail: russull.rushdi@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2669

ABSTRACT: Graphene oxide (GO) nanosheets were prepared by a novel simplified Hummer's method. The morphological and cross section images of GO have been tested with field emission-scanning electron microscope (FE-SEM). The antibacterial activity of GO nansheets against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were investigated as a model for Gram-negative bacteria and Gram-positive bacteria respectively. Bacteriological tests were performed by agar well diffusion assay with different concentrations of GO nanosheets and the bacterial morphological change of two bacterial species has been studied by scanning electron microscope (SEM) before and after treated with GO nanosheets. These sheets have been approved to be an effective bactericide. The antibacterial activity of the nanosheets dispersion was measured by agar well diffusion method. Scanning electron microscopy (SEM) was used to investigate the biocidal action of this nanoscale material. The nanosheets of GO have shown a high antibacterial activity against Gram-positive bacteria. The results of the present work offer a novel assay to prepare GO nanosheets were it could be used as novel antibacterial agent in future for different areas of biomedical and pharmaceutical sciences, like biosensing, antibiotics, imaging, and drug delivery.

KEYWORDS: Agar Well Diffusion Assay; Antibacterial Activity; Graphene Oxide; SEM

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