Extraction of Protease Under Solid State Fermentation Using Bacterial Isolates from Traditional Leather Processing Waste Water Found Around Wukro Maray

Girma Haile and Birhanu Babiye

Ethiopian Institute of Agricultural Research, National Agricultural Biotechnology Research Center, Microbial Biotechnology Program.

Corresponding Author E-mail: bbirhanu23@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2656

ABSTRACT: Enzymes are important in reducing both energy consumption and combating environmental pollution. Proteases are enzymes which catalyze the hydrolysis of protein molecules.Most of the tannery industries in Ethiopia use chemicals for dehairing that led great environmental and human health problem. The objectives of the present study were,to isolate potential protease producing bacteria from water sample collected from traditional leather processing waste water around Wukro maray;to extract the protease enzyme through SSF using cheap wheat bran, and evaluate the potential activity of the enzyme in leather dehairing. Water samples were serially diluted and 1ml of sample was spread on nutrient agar and kept at 37⁰C for 24 hrs. Many colonies of bacteria were formed. The colony from C10^-4 and G10^-3 were taken by using inoculating loop for sub culturing to get pure colony. Then the pure cultured colony were inoculated into the 250 ml Erlenmeyer flasks containing substrate were fermented after 6 days incubation at 37⁰C. The results of the unknown concentration of the crude protease enzyme showed successfully used as dehairing agent on hide. The results indicate that these bacteria isolate can be used as biotechnological tool for industrial purpose.

KEYWORDS: Bacteria; Leather Dehairing; Protease; Solid State Fermentation (SSF); Wastesq

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