Phylogenetic Assessment of Garcinia Species Using RAPD Markers

Madappa Machamada Bheemaiah¹, Bopaiah Ajikuttira Kushalappa² and Grace Prabhakar³

¹Development Centre, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India.

²Department of Botany, St. Joseph’s College (Autonomous), No 36, Lalbagh Road, Shanthinagar, Bengaluru, 560027, Karnataka, India.

³Department of Biotechnology, St. Joseph’s College (Autonomous), No 36, Lalbagh Road, Shanthinagar, Bengaluru, 560027, Karnataka, India.

Corresponding Author E-mail: madappa@sjc.ac.in

DOI : http://dx.doi.org/10.13005/bbra/2706

ABSTRACT: The plants in the Garcinia species are economically important. Phylogenetic investigation is needed for these tree species to boost breeding and conservation programmes. Six Garcinia species were investigated for their phylogenetic relationship using Random Amplified Polymorphic DNA(RAPD) markers. A standardised procedure was developed for isolation of DNA from the leaf samples of G. cambogia, G. indica, G. xanthochymus, G. morella, G. mangostana and G. livingstonei. Phylogenetic investigation is needed for these tree species to boost breeding and conservation programmes. A standardised procedure was developed for isolation of DNA from the leaf samples of G. cambogia, G. indica, G. xanthochymus, G. morella, G. mangostana and G. livingstonei. The DNA samples were subjected to PCR using 8 random primers. 269 polymorphic bands were obtained and scored to develop the values for the genetic distance. The dendrogram was developed using the software dendroUPGMA and the Cophenetic correlation coefficient of 0.801 is obtained. G. cambogia and G. livingstonei are closely placed with a score of 24% followed by G. morella. It had a 30% index score to G. cambogia and G. livingstonei but is followed by just 31% score with G. indica. G.mangostana is connected at 33.5% dissimilarity to the above groups showing it is an introduced variety. G. xanthochymus is the last link with 37% score in the matrix. The data represented is the first of the type for the species. This will help in further DNA related work in these species. The genetic relatedness among these species is reported and this can be utilised in marker analysis for other Garcinia species.

KEYWORDS: Garcinia; RAPD; Phylogenetic Analysis

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