Formulation of A Suitable Medium and its Optimization for Maximizing L-Asparaginase Production from Endophytic Fungi Fusarium Sp. LCJ273

Antony V. Jenila and J. Joel Gnanadoss

Microbial and Environmental Biotechnology Research Unit, Department of Plant Biology and Biotechnology, Loyola College, Chennai 600 034, Tamil Nadu, India.

Corresponding Author E-mail: joelgna@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2699

ABSTRACT: Endophytic fungi Fusarium sp. LCJ273 capable of producing L-asparaginase was isolated from the medicinal plant Adhatoda vasica. The aim of the present study was to maximize L-asparaginase production by submerged fermentation through statistical optimization. L-Asparaginase production by Fusarium sp. LCJ273 was studied in five different media. Various nutritional parameters specifically carbon, nitrogen and inducers were optimized for enhancing the production of L-asparaginase. In addition, different statistical based experimental designs were also applied to increase the production of L-asparaginase by Fusarium sp. LCJ273. Dextrose, ammonium sulphate and wheat bran were found to be effective for growth and higher yield of L-asparaginase in Modified Czapek’s Dox Broth. Dextrose at a concentration of 3.0 g/L increased L-asparaginase production up to 9.18±0.9 U/mL, ammonium sulphate at the concentration of 20 g/L showed maximum L-asparaginase production up to 13.69±0.4 U/mL and wheat bran at 2.5 g/L yielded up to 14.24±0.5 U/mL. The maximum L-asparaginase production was observed by Fusarium sp. LCJ273 on 5^th day. The study revealed that through optimization, a 2 fold increase in L-asparaginase could be achieved.

KEYWORDS: Endophytic Fungi; Enzyme Production; L-Asparaginase; Submerged Fermentation

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