miRNA-1, miRNA-145 As A Myocardial Infarction Diagnostic Biomarker

Ibrahim Abdul-Majeed Altamemi¹, Aqeel Raheem Hassan² and Alawi Jawad³

¹Department of Medical Microbiology, College of Medicine, University of  Al-Qadisiyah.

²Department of Medicine, College of  Medicine, University of Al-Qadisiyah.

³M. B. CH. B Resident Physician at Al-Dewaniyah Teaching Hospital.

Corresponding Author E-mail: ibrahim.altamemi@qu.edu.iq

DOI : http://dx.doi.org/10.13005/bbra/2712

ABSTRACT: Many myocardial infarction biomarkers currently available but they are a lack of specificity, therefore present study suggests to evaluate the significant importance of miRNA-1, miRNA-145 as biomarkers for early diagnosis of myocardial infarction. A blood sample was collected from three groups. The first group was patients with acute myocardial infarction (MI), the Second group was  patients who have a risk factor for MI, and the Third group included  healthy volunteers. Serum blood of this sample used to RNA purification and cDNA application with stem-loop specific primer then miRNA-1, and miRNA-145 was quantitated by using RT-PCR. The level of miR-1 fold change was significantly highest in the MI group followed by risk group and then by control group (P<0.05). while of miRNA-145 fold change was significantly lowest in the MI group followed by risk group and then by control group (P<0.05). A receiver operator characteristic (ROC) analysis; the cut off value was identified at miRNA-1 of >5.28 fold change with a sensitivity of 91.67 % and a specificity of 90.7%, while  the cut off value of miRNA-145 has cut off ≤ 0.7 fold change with a sensitivity of 95.83 % and a specificity of 89.47%. miRNA-1, miR145 has high sensitivity and Specificity in this study which was bushed to using them as an alone biomarker or supported for Another biomarker in AMI diagnosis.

KEYWORDS: miRNA-1; miRNA-145; MI

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