Proteomic Analysis of GA3-Induced Berry Elongation in Grape (Vitis Vinifera L.) Cultivar Thompson Seedless

Smita Maske-Ghule, Anuradha Upadhyay* and Satisha Jogaiah

ICAR-National Research Centre for Grapes, Solapur Road, Pune, Maharashtra 412307, India.

Corresponding Author E-mail: anuradha.upadhyay@icar.gov.in

DOI : http://dx.doi.org/10.13005/bbra/2724

ABSTRACT: GA₃ is a commonly used plant growth hormone for berry elongation in grapes. However, the basic molecular mechanism behind GA₃ mediated berry-sizing is unclear. In current study, bunches of Thompson Seedless (Vitis vinifera L.) were sprayed with 25 ppm GA₃ at 3-4 mm berry stage and berry samples were harvested at 6 h, 24 h, and 48 h after application. Among the detected 1935 proteins, 129 proteins were successfully identified with significant abundance at least one application time point. These proteins were assigned to carbohydrate metabolism, primary amino acid metabolism, photosynthesis related proteins, citric acid cycle, phenylpropanoid, and defense related pathways. Consequently, current study offers the identification of GA₃ responsive proteins at berry stage and assesses the time course of proteome modulation during berry elongation by using proteomic approach.

KEYWORDS: Berry Elongation; Grape; Gibberellic Acid; Proteome Analysis

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