Seroconversion Studies of Indian Newcastle Disease Virus Isolates of Genotype XIII in 3 Week Old Chickens

S. J. Jakhesara¹*, A. K. Patel², Priya Malsaria², J. K. Pal² and C. G. Joshi¹

¹Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, 388001- India.

²Hester Biosciences Limited, At and Po.: Merda-Ardej, Tq.: Kadi, Dist.: Mehsana-382721, Gujarat, India.

Corresponding Author E-mail: drsubhash81@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2717

ABSTRACT: Poultry industry plays an important role in sustaining economy of India. Despite routine vaccination strategies has been a common practice to control commonly occurring diseases in poultry, outbreaks are commonplace. Recently, emergence of genotype XIII strains of NDV resulted in widespread economic losses in India. We prepared inactivated oil adjuvant vaccine derived from Lasota, ndv53/Haryana or ndv52/Sarsa, which is recently isolated genotype XIII virus in India. Three groups of SPF chickens were vaccinated once with each vaccine and serum samples were collected every 7 days interval and tested for HI titres with three different antigens prepared from same virus to assess cross neutralization antibodies amongst them. All three vaccines have shown some degree of cross reactivity after 14 days post vaccination. However, vaccine prepared from Lasota, a genotype II virus failed to generate significant titers against both ndv53/Haryana and ndv52/Sarsa. Our observation explains recent outbreaks of genotype XIII viruses in the field and necessitates development of new vaccines to control the recently emerged NDV strains in India.

KEYWORDS: Haemagglutination Test; Newcastle Disease Virus; Seroconversion; Vaccine

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