Molecular Identification, Extracellular Enzyme Production and Antimicrobial Activity of Endophytic Fungi Isolated from Solanum Tuberosum L. in Egypt

Yasser M. M, Mousa A. S. M, Marym A. Marzoukand Tagyan A. I*

Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Egypt.

Corresponding Author E-mail: i_aya50@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2731

ABSTRACT: Solanum tuberosum L. possesses economic properties and can host endophytic mycoflora. A total of 19 endophytic fungi were identified via morphological and molecular methods. Among them, Trichoderma harzianum was the core-group fungus with a relative frequency of 36.7%. In the preliminary antimicrobial assay, all the test pathogens were inhibited by Alternaria tenuissima, Penicillium pinophilum and Penicillium rubens with a maximum inhibition zone of 26 mm and a minimum zone of 11 mm using agar-plug method. All the isolated endophytic fungi produced amylase, while cellulase and tyrosinase were recorded for most of the isolated species, whereas laccase and protease and manganese peroxidase were shown by a few taxa. None of the isolated fungi produced chitinase. This study revealed the biodiversity of endophytic fungi isolated from Solanum tuberosum that could be a promising source of bioactive compounds applied in many industries.

KEYWORDS: Biodiversity; Endophytic Fungi; Solanum tuberosum; Trichoderma harzianum

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