Association of +10211T/G (Rs17846866) Variant of Adiponectin Gene With Type 2 Diabetes Mellitus

Mohammad Mustufa Khan¹ and Roshan Alam^2*

¹Department of Biomedical Sciences, School of Biological Engineering and Sciences, Shobhit University, Gangoh, Saharanpur, Uttar Pradesh, India- 247341

²Department of Biochemistry, Integral Institute of Medical Sciences and Research, Integral University, Lucknow, Uttar Pradesh, India- 226026

Corresponding Author E-mail: dr.roshan.alam@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2772

ABSTRACT: Various adiponectin gene (ADIPOQ) variants, located on chromosome 3q27 were associated with Type 2 diabetes mellitus (T2DM) in different ethnicity.In this study, it is aimed to find the association of +10211T/G (rs17846866) variant of ADIPOQ with T2DM and healthy controls in North Indians.In this study, 150 T2DM and 150 healthy control subjects aged between 25-75 years were recruited. Circulatory adiponectin levels were measured by commercially available ELISA kit. For genotype analysis, Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) method was used.The genotypic analysis of rs17846866 variant of ADIPOQ has shown that there were no significant association of TT versus TG genotype (P=0.13) as well as TT versus GG genotype (P=0.11) with T2DM patients and healthy controls. However, the G allele frequency of the rs17846866 has shown significant association with T2DM (13.7%) as compared to healthy controls (7.7%, P=0.02). In T2DM, circulatory adiponectin level was significantly lower in TT genotype than TG genotypes (P=0.01). However, the circulatory adiponectin level was lower in GG genotype than TG genotype (P=0.49), but not significant.The result showed that rs17846866 variant of ADIPOQ was associated with altered circulatory adiponectin levels. The TT genotype may be the major contributor to reduce the circulatory adiponectin levels in T2DM. However, the G allele may be increased the risk of T2DM in North Indians.

KEYWORDS: ADIPOQ; Circulatory adiponectin; rs17846866; Type 2 diabetes; variant; Type 2 diabetes

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