Immunological Activities of Isoprinosine Inhibition on Viral Infections in Human

Hashim Ali Abdualmeer Al-sherees*, Sumaya Najim Abedali Al-khateeb and Fadhil Hussain Nasir Al-Muhannak 

Department of Microbiology, Faculty of Medicine, Kufa University, Kufa

Corresponding Author E-mail: hashimaa49@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2793

ABSTRACT: Isoprinosine is a combination of inosine used as antiviral drug without effect on viral particle itself, but instead only and acts as on immunostimulant and also acts indirectly by activation of immune cells. Aim of this study was to determine level of interferon-alpha (INF-α) with parainfluenza viruses HPIV-2, and adenoviruses HAdV-2 replication. In the present study, cytotoxic effect of isoprinosine was assessed using A549 cell line exposed to different concentrations of compound (isoprinosine: 50-800μg/mL) for 48 hours. Cytotoxic effect was examined visually using light, inverted microscopy Olympus CK2 under 400x magnification and by the MTT colorimetric assay. The yield re­duction assay (YRA), which evaluates the ability of the isoprinosine (50-800 μg/mL) to inhibit virus multiplication in cell cultures, was applied. The cytopathic effect of the virus was evaluated 48 h after infection of A549 cell cultures with viruses by means of light, inverted microsco­py. The YRA method was used to determine the 50% end point (IC₅₀) in the presence of Isoprinosine with the controlled one. MTT cytotoxicity assay confirmed microscopic observations. There were no morphological changes, as assessed visually, in cell cultures treated with isoprinosine. After conducting the experi­ments and analyzing the results we noticed that higher concentrations of isoprinosine strongly inhibited multiplication of all viruses. HPIV-2 and HAdV-2 showed the highest sensitivity to the antiviral activity of isoprinosine as compared with the control, however, increasing concentrations of isoprinosine up to 800 μg /ml slightly enhanced the antiviral activity of 400 μg/ml isoprinosine. Our study was conducted that HAdV-2 and HPIV-2 have the highest sensitivity to the antiviral activity of isoprinosine from all tested viral strains.

KEYWORDS: Adenoviruses; Antiviral Activity; Isoprinosine; Parainfluenza Viruses

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