The Antibacterial and Coagulant Activity of Psidium Guajava Leaves Extracts in Purification of Wastewater

Marwa E. El-Sesy^1*and Belal N.A.Mahran²

¹Microbiology Department, Central Laboratory for Environmental Quality Monitoring (CLEQM), National Water Research Center (NWRC), Cairo, Egypt.

²Organic Department, Central Laboratory for Environmental Quality Monitoring (CLEQM), National Water Research Center (NWRC), Cairo, Egypt.

Corresponding Author E-mail : marwa.micro@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2823

ABSTRACT: This study expounded the ability to use Psidium guajava leaves extract as coagulant, fecal coliform removal and for decreasing values of some heavy metals. The optimum dosages of Psidium guajava leaves extracts needed for treatment the collected wastewater samples were studied. The Physico-chemical characterization and bacteriological analysis for wastewater samples were determined before and after treatment. Results demonstrated that the turbidity removal is observed up to 73 % and fecal coliform removal about 97% using 1.5 mg/L of Psidium guajava leaves ethanol extract. There is a marked decrease in the values of some heavy metals as copper, zinc, chromium, cadmium and lead values. The antimicrobial activity of leaves extracts against some pathogenic bacteria using disc diffusion method and viability test was examined, revealed bactericidal effect on Staphylococcus aureus, Bacillus cereus and E. coli. Studying the ultra-structure of E. coli and Bacillus cereus cells treated with MICs extracts of Psidium guajava leaves revealed the deformation of cells accompanied with rupture of cell membranes at certain sites and leakage of cellular contents. Using gas chromatograph Mass Spectrometry (GC-MS), to identify chemical compositions of Psidium guajava leaves extract and their bioactive components indicate the presence of β-Caryophyllene and Alpha-Bisabolol that reveled the biological activities of Psidium guajava as Antimicrobial. Psidium guajava leaves extract seems to be environmental friendly and an effective agent in treatment wastewater.

KEYWORDS: Antibacterial Activity; Extracts; Heavy Metal, Gc-Ms; Psidium Guajava; Wastewater

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