Molecular Characterization of Thirteen Oil seed Brassica L. Variants From Bangladesh Through Polyacrylamide Gel Electrophoresis (PAGE)

Faria Akbar, Susmita Saha, Meghla Saha Pinky and Kazi Nahida Begum*

Department of Botany, Faculty of Life and Earth Sciences, Jagannath University, Chittaranjan Avenue, Dhaka-1100, Bangladesh

Corresponding Author E-mail: kazinahida@bot.jnu.ac.bd

DOI : http://dx.doi.org/10.13005/bbra/2879

ABSTRACT:

Brassica L. is the most agronomical important genus of Brassicaceae family. An electrophoretic exploration was conveyed for proper identification of genetically diverse and agronomically superior genotypes and pursuing the extent of genetic divergence and phylogenetic relationship within the thirteen variants of Brassica for leaf storage protein by using Polyacrylamide Gel Electrophoresis (PAGE) as biochemical marker. A total of 19 alternative protein bands were found with high polymorphism of 89.47%. The protein banding pattern suggested the existence of differences among the studied variants pertaining to the location, molecular weight and staining intensity of the bands which could be utilized as fingerprints for variants identification. Based on Nei’s genetic distance, a wide range of genetic distance (0.0541–1.5581) offered the presence of broad genetic variability among the quested variants. A dendrogram was constructed by using UPGMA where all the analyzed Brassica variants were rouped into two major clusters. Relying on this analysis, highest genetic variation (1.5581) was observed between BS-10 and BS-14 while the lowest genetic variation (0.0541) was recorded between BS-9 and BS-12, which might be furnished as a source of parental line. Consequently, it can be proposed that the protein profile of the analyzed thirteen variants of Brassica L. by PAGE would be considered to be a contributory implement to the breeders of Brassica by providing sufficient information on the genetic resources of Brassica and improvement of new offspring in the forthcoming breeding program of Brassica L.

KEYWORDS: Brassica L; Genetic diversity; Leaf protein; PAGE

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