GC-MS Profiling and Antifungal Activity of Secondary Metabolite from Endophytic Fungus of Giloy

Akanksha Raj Sriwastava and Vivek Srivastava*

Department of biotechnology, Rama University Kanpur, India.

Corresponding Author E-mail: viveksrivastavabio@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2948

ABSTRACT:

The endophytic microbiota is considered to be one of the consistent and noble souce of potential and unique natural amalgams. These natural amalgams carry diverse pharmaceutical significance which the reason for their importance among research fields. The diversity of plants carries much more diversity of the endophytes as their mutual parts where both are benefited from each other. The current work deals with the isolation of the endophytic fungus from Tinospora cordifolia, for which the leaves were used after the surface sterilization, followed by the production of secondary metabolite by the endophytic isolates through submerged fermentation technique. The produced metabolite was extracted by liquid-liquid extraction technique, which was further used for evaluating its antifungal potential against Candida albicans and the obtained results show their considerable potential. The GC-MS profiling of secondary metabolite was conducted to determine the presence of some bioactive compounds in them, and as a result, some potential compounds detected are Levoglucosenone, Silanediol, Nonane, D-Allose, 5-Hydroxymethylfurfural. Since these compounds are biologically important in various aspects which gives the diversified application to the secondary metabolites. The study concludes the potential of secondary metabolites from endophytic fungus of Tinospora cordifolia and further investigation can be approached on determining the same from other plants, and also evaluating another bioactive potential of secondary metabolites.

KEYWORDS: Endophytic fungus; GC-MS analysis; Secondary metabolite; Tinospora cordifolia

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