Studies on the Actions of Calcium Channel Blockers, Anticholinesterases Agents on Experimentally Induced Spastic Paralysis in Normotensive Rats

P. I Aziba^1*, Tari R. Idumange¹, Senjobi, C. T², Adeniyi-Akee M³ and Adedokun Oluwasegun⁴

¹Department of Pharmacology and Toxicology, Dora Akunyili College of Pharmacy Igbinedion  University, Okada  Olabisi  Onabanjo University, Ago Iwoye Nigeria.

²Department of Plant science,  Igbinedion  University, olabisi onabanjo university Ago Iwoye. Nigeria.

³Department of Pharmaceutical Chemistry, Igbinedion  University, Okada Olabisi  Onabanjo University, Ago Iwoye Nigeria.

⁴Department of  Pharmacognosy, Igbinedion  University, Okada Olabisi  Onabanjo University, Ago Iwoye Nigeria.

Corresponding Author E-mail: peteraziba1@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2991

ABSTRACT:

Experimental paralysis was induced intramuscularly (i.m) in rats , using Suxamethonium(1.08mg/kg ) , Decamethonium (1.08mg/kg ) in normotensive rats weighing150-180g±2.5g (n=8).Various concentrations of these drugs ranging from (0.36—1.08mg/kg) were used , in order to assess at what concentrations will induced spastic paralysis sustained without  death. Similarly, dose response concentrations effect was examined and the onset of action( ie time taken to observe paralysis and manifestation of pain and death was recorded. In some experiments, equimolar concentrations  of Suxamethonium(1.08mg/kg) and Decamethonium(1.08mg/kg) was used to assess reaction time(RT) and potency of the agents. Thereafter the actions of  Calcium Channel blockers (CCB), Diltiazem ( DTZ) and verapamil(VE) were studied in separate experiments. Anticholinesterase agent(AchE) in combination with Atropine a known antimuscarinic agent(AchE )  and investigated  their actions on induced paralysis. The results obtained in these studies showed that CCB produced a significant potentiation of spastic paralysis on Suxamethonium induced paralysis but non reversal effect with Decamethonium , while  AchE in combination ATR showed a reversal effect on paralysis induced by these agents in the concentrations used in his study. The aim of the study was to see interaction of these agents on sustained experimental paralysis in rodent.

KEYWORDS: Anticholinesterases; Calcium Channel blockers; Neuromuscular  depolarizing; Paralysis

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