Response surface methodological approach to optimize the nutritional parameters for lipase production by Aspergillus japonicus MTCC 1975 under solid state fermentation

I. Sarat Babu¹*, K. Sita Kumari², V. V. Sridevi³ and M. N. Rao³

¹Department of Biotechnology, A.N.I.T.S, Sangivalasa, Bheemunipatnam, Visakhapatnam - 531 162 (India). ²Department of Pharmaceutical sciences, M.R.P.G.College, Phool Bagh, Vizianagaram - 535 002 (India). ³Center for biotechnology, Department of chemical engineering, Andhra University, Visakahapatnam - 530 003 (India).

ABSTRACT: Lipase production was optimized by Aspergillus japonicus MTCC 1975 in solid state fermentation using sugarcane bagasse and wheat bran as the mixed substrate. Response surface methodology was used to achieve the optimization of the experimental conditions for the optimal production of lipase. To study the proposed second order polynomial model, the central composite experimental design with multiple linear regression was used to estimate the model coefficients of the three selected factors. These factors were considered to influence the optimization process. The best yields were obtained at pH of 7, substrate of 10 g, and 80 % of moisture content. This methodology was found to be very efficient and only 20 experiments were needed to assess these conditions. The model adequacy was very satisfactory, as the coefficient of determination was 0.9085.

KEYWORDS: Central composite design; lipase; response surface methodology

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