Purification and Structural Characterization of an Antimicrobial Compound, Lipoxazolidinone a Produced by a Lactobacillus Apis YMP3

Dayanidhi Satish Kumar ^1,2 and Palanisamy Venkatachalam ^1*

¹Department of Microbiology, Sengunthar Arts and Science College (Affiliated to Periyar University), Tiruchengode – 637205, Tamil Nadu, India.

²Department of Microbiology, Indira Gandhi College of Arts and Science (Affiliated to Pondicherry University), Kathirkamam – 605009, Puducherry, India.

Corresponding Author E-mail: venmalar.2007@rediffmail.com

DOI : http://dx.doi.org/10.13005/bbra/3090

ABSTRACT: Strains of Vibrio cholerae are one among the most causative and serious disease causing human pathogenic agents, its infections are caused mostly by ingesting contaminated water and/or food. According to the recent estimates, between 1.3 and 4.0 million individuals are infected all around the world every year. The lactic acid bacteria are an important class of probiotics microbes have their ability to produce diversified bioactive compounds, hence this study focused on the identification of a promising antimicrobial agent from a Lactobacillus apis YMP3. This strain was cultured on MRS broth and the cell free supernatant was ethyl acetate extracted for the antimicrobial agent. The crude extract was further purified with C₁₈ silica gel column chromatography and structurally characterized by FT-IR, NMR, GC and MS/MS spectrum. The chemistry of the compound was confirmed as Lipoxazolidinone A which has the IUPAC name of (2E)-5-butyl-2-[(E)-4-methyl-2-oxoundec-3-enylidene]-1,3-oxazolidin-4-one. This is the first report of Lipoxazolidinone A produced by a bacterium, L. apis YMP3 which was originally isolated from yoghurt. This finding expands the scope of identifying more promising bioactive compounds from probiotic Lactobacillus sp., further, this systematic procedure for purification of this antimicrobial agent stood as the baseline data for more elaborate therapeutic studies in future.

KEYWORDS: Antimicrobial compound; V. cholerae; Lipoxazolidinone A; Lactobacillus apis; Purification; YMP3

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