Investigations and Characterization of Alkaline Protease-Producing Fermentibacillus sp. RSCVS-HS3

Rakesh Singh Chauhan^1*and Rahasya Mani Mishra^1,2

¹Centre for Biotechnology Studies, Awadhesh Pratap Singh University, Rewa, 486003, Madhya Pradesh, India.

²Department of Environmental Biology, Awadhesh Pratap Singh University, Rewa, 486003, Madhya Pradesh, India.

Corresponding Author E-mail: rakesh.s.chauhan1@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/3093

ABSTRACT: The study's goal was to investigate and characterize alkaline protease-producing Fermentibacillus sp. RSCVS-HS3. The bacterium Fermentibacillus sp. RSCVS-HS3 was isolated from Vindhya region (Rewa division) of Madhya Pradesh of central India. It grew on casein-containing media at pH 12 and protease activity was found positive. . The enzyme was in a stable state in its crude form at 50℃ and pH 12, indicating thermal stability and the alkaline nature of the enzyme. It was rod-shaped, long, filamentous, gram-positive bacterium and was positive for casein hydrolysis. Based on NCBI BLAST and Phylogenetic Analysis of 16s rRNA, it was identified as Fermentibacillus sp. RSCVS-HS3. This is probably the first study to date, stating the alkaline protease synthesis from any Fermentibacillus species. It was closest to Fermentibacilolus polygoni IEB3 but in contrast to this, the identified bacterium showed casein hydrolysis. This is probably the first study to date, stating the alkaline protease synthesis from any Fermentibacillus species.

KEYWORDS: Alkaline protease; Bacteria; Characterization; Fermentibacillus; Identification; Soil

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