Investigating the Antibacterial Properties of Silver Nanoparticles Acquired using Streptomyces strain AK3 from Riverbank Soil

Arun Kumar Kulshrestha* and Priti Hemant Patel

Department of Biotechnology, Ganpat University, Gujarat, India.

Corresponding Author E-mail: arunmonty368@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/3094

ABSTRACT: The soil sample was acquired from a heavily metal polluted site on the Tapi River in Surat, Gujarat, India, diluted serially, and dispersed over an actinomycetes isolation medium. Isolates were cultured in 100 ml of starch-casein broth at 30⁰ C for 72 hours in an incubator with shaking. The cell-free filtrate was added to a final solution of 1 mM silver nitrate, which was then dried at 250⁰ C. Using a spectrophotometer, silver nanoparticles were quantified, data on size distribution and zeta potential were acquired from Malvern, and the 16S rRNA gene was amplified in a PCR mixture. As a result of the addition of silver nitrate to the S. atacamensis strain AK3 filtrate, the reducers altered the broth's color from yellow to light brown. The highest absorbance was measured at 420 nm, and the 0.25 polydispersity index was below the agglomeration threshold. The TEM indicated their spherical to ellipsoidal shape and 20 nm size. The NJ approach to sequence alignment revealed that the strain was 99.42% similar to S. atacamensis C60. Zones of inhibition of S. epidermidis, A. baumanni, N. gonorrhoeae, and L. monocytogenes were found to be 18±1 mm, 19±1 mm, 20±1 mm, and 14±1 mm respectively, at 35 μg/ml AgNPs, proving the efficiency of AgNPs synthesized by the strain.

KEYWORDS: Antibacterial activity; Silver nanoparticles; TEM; UV-Vis spectra; Zeta potential

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