Chlorpyrifos Degradation by Bacillus tropicus a Plant Growth Promoting Rhizobacteria

Deepak KumarMalik^1*, Raina Singhmar¹ , Vivek Singh¹, Meenu Rathi² and Vishal Ahlawat³

¹Department of Biotechnology, University Institute of Engineering and Technology, Kurukshetra University, Kurukshetra, Haryana, India.

²Department of Botany, Gandhi Memorial National College, Ambala Cantt, Haryana, India.

³Department of Mechanical Engineering, University Institute of Engineering and Technology, Kurukshetra University, Kurukshetra, Haryana, India.

Corresponding Author E-mail: dmalik2015@kuk.ac.in

DOI : http://dx.doi.org/10.13005/bbra/3230

ABSTRACT: Excessive use of organophosphate in modern farming to improve the crop productivity has cause pollution in soil, water and air which lead environmental as well as human hazards. Microbial fertility is adversely affected by the use of pesticides. Thus, the present study focused on the isolation and screening of effective isolates with multi-traits PGPR activities and further studied for chlorpyrifos pesticide degradation. The bacterial isolate DK5 was showing multiple PGPR activity, identified as Bacillus tropicus by 16S rRNA sequencing. The chlorpyrifos degradation by isolated Bacillus tropicuswas studied by using resting cell study. In HPLC analysis revealed that Bacillus tropicus degrade 60% chlorpyrifos after 48 hrs. of incubation followed by 99% after 72 hrs. of incubation. Generally, results of this study revealed that isolate DK5 identified as Bacillus tropicus can be used for the successfully removal of chlorpyrifos from contaminated soil and plant growth promotion.

KEYWORDS: Chlorpyrifos; HPLC; Pesticide; PGPR

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