Antioxidant activity of flavonoids isolated from Dolichos biflorus Linn. in rabbits fed high fat diet

A. KottaiMuthu¹*, S. Sethupathy², R. Manavalan¹ and P.K. Karar¹

¹Department of Pharmacy, Annamalai University, Annamalai Nagar - 608 002, (India) ²Division of Biochemistry, Rajah Muthiah Medical College and Hospital, Annamalai University, Annamalai Nagar - 608 002 (India)

ABSTRACT: Flavonoids from Dolichos biflorus Linn. were isolated and screened for antioxidant activity on rabbits fed high fat diet (HFD). Methanolic extract of Dolichos biflorus was fractionated with hexane, chloroform, ethylacetate and methanol. Ethylacetate fraction which showed maximum flavonoid yield, were fractionated by column chromatography and estimated for flavonoids (total phenol) content. High fat diet rabbits showed significant decreased activities of tissue enzymatic and non enzymatic antioxidant. High fat diet induces the oxidative stress in cell by producing reactive oxygen species. Antioxidant Enzymes such as Superoxide dismutase, Catalase, Glutathione peroxidase, Glutathione-s-transferase, and non enzymatic antioxidant Glutathione showed enhanced activities on administration of isolated flavonoids from Dolichos biflorus in high fat diet rabbits. Hence it is concluded that flavonoids isolated from Dolichos biflorus were found to have protective action against high fat diet induced oxidative stress in different tissues in rabbits.

KEYWORDS: Antioxidant activity ; Dolichos biflorus; flavonoids; high fat diet; rabbits

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