In vitro evaluation of antioxidant properties and their capacity of Alternanthera sessilis and Tephrosia pupurea

D. Bhaskar Rao, P. Subhashini Devi, P. Aparanji, M. Rama Rao* and T. Raghava Rao

¹Department of Biochemistry, College of Science and Technology, Andhra University, Vishakhapthanam - 03 (India) ²Department of Biochemistry, College of Science, GITAM University, Vishakhapthanam -45 (India)

ABSTRACT: Currently, there is a great interest in functional components of food ingredients. This is mainly due to the fact that many studies have shown that reactive oxygen species (ROS), including oxygen free radicals, are a part of etiology of degenerative disorders including some hepatopathies and other serious organ damage. Aqueous extracts of Alternanthera sessilis and Tephrosia purpurea were screened for potential antioxidant properties, which includes various enzymatic antioxidants like superoxide dismutase, catalase, peroxidase, glutathione peroxidase and ascorbate oxidase and various non-enzymatic antioxidants such as reduced glutathione, vitamin C and total phenolics. These plants extracts also analysed for in vitro antioxidant capacity assay, which includes the total antioxidant power, reducing power and the radical scavenging assays. The results clearly indicate that both the plants of the present study are beneficial as an antioxidant sources since both the plants possesses significant levels of enzymatic antioxidants, non-enzymatic antioxidants and also exhibits antioxidant capacity.

KEYWORDS: Free radicals; antioxidants; Alternanthera sessilis; Tephrosia purpurea

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