Identification of Wild Type Streptococcus Zooepidemicus and Optimization of Culture Medium and Fermentation Conditions for Production of Hyaluronic Acid

Alireza Zakeri¹, and Mohammad Javad Rasaee¹*    

¹Department of Medical Biotechnology, School of Medical Sciences,Tarbiat Modares University,Tehran,Iran Corresponding author email:rasaee_m@modares.ac.ir

DOI : http://dx.doi.org/10.13005/bbra/2022

ABSTRACT: Strptococcusequi subsp. Zooepidemicus (S. zooepdemicus) is among the hyaluronic acid (HA) producing bacterial strains. Due to its pervasive applications in medicine and cosmetics, HA is an economically valuable product. Therefore, charactering a novel high yield HA producing strain seems to be industrially appealing. In this regard we characterized a novel strain of S. zooepdemicus using. Thereafter, the amenable carbon and nitrogen sources were determined and optimized using one factor at a time approach. To have the highest yields of HA production during the fermentation, Taguchi orthogonal arrays were used to design 9 experiments. Our results revealed that glucose (30g/L) and yeast extract (70g/L) are the best carbon and nitrogen sources respectively. Moreover, according to our results the optimum culture conditions regarding the temperature, DO, rotate speed and pH were 37⁰C, 50%, 300 rpm and 7 respectively. The prioritized order of factors affecting HA production were as: temperature>DO > rotate speed> initial pH.The 103.84% increase in HA production under the optimum culture medium and conditions approves the robustness of the adopted approach. Since the wild type strain demonstrated high intrinsic capability of HA production in optimum conditions, this novel strain could be employed for large scale production of HA.

KEYWORDS: S. zooepdemicus; Hyaluronic acid; Taguchi orthogonal arrays; Fermentation

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