Volume 5, number 1
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Utilization of pomegranate peel waste for the production and characterization of invertase using Cladosporium sp.

C. Uma* and V. K. Gopalakrishnan

Department of Biochemistry, Karpagam Arts and Science College, Coimbatore - 641 021 India.

ABSTRACT: Invertase production by Cladosporium sp., at varying pH, temperature, period of incubation was studied by using Czapek Dox as basal medium. Sucrose in the medium was substituted with pomegranate peel waste. The effect of metal ions of invertase was were also carried out. The results of the study revealed that the production of invertase was maximum on the 5th day of incubation at an optimum pH of 6.0 and optimum temperature of 300C. Purification of invertase was carried out using ammonium sulfate, dialysis followed by acetone precipitation. Protein content and thermal stability of the purified invertase was determined.

KEYWORDS: Invertase; Pomegranate peel; Cladosporium sp.

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Uma C, Gopalakrishnan V. K. Utilization of pomegranate peel waste for the production and characterization of invertase using Cladosporium sp. Biosci Biotechnol Res Asia 2008;5(1)
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Uma C, Gopalakrishnan V. K. Utilization of pomegranate peel waste for the production and characterization of invertase using Cladosporium sp. Biosci Biotechnol Res Asia 2008;5(1). Available from: https://www.biotech-asia.org/?p=6741

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