Enhanced shoot organogenesis in Aloe saponaria following treatment with ethylene inhibitors and polyamines

Sook Young Lee¹, Thanislas Bastin Baskar², Jae Kwang Kim³ and Sang Un Park^2*

¹Regional Innovation Center for Dental Science and Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju, 501-759, Korea ² Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon, 305-764, Korea ³Division of Life Sciencesand Bio-Resource and Environmental Center, Incheon National University, Incheon 406-772, Korea

DOI : http://dx.doi.org/10.13005/bbra/1997

ABSTRACT: Plants belonging to the genus Aloe are wide spread in Africa and are important in the pharmaceutical industry. Here, we investigated the role of ethylene inhibitors and polyamines in the enhancement of shoot regeneration from meristem explants of Aloe saponaria. A rapid, highly effective shoot regeneration procedure was designed using meristem explants. First, we found that Murashige and Skoog (MS)with 2mg/L6-benzyl amino purine (BAP) was the best for shoot initiation. Shoot regeneration was assessed using different concentrations of AgNO_3,aminoethoxyvinylglycine (AVG), and CoCl₂ (1, 5, 10, and 20mg/L).While AgNO₃ induced shoots (3.5/explant) and increased shoot length by 2.5cm at concentrations of 10mg/L, an additional increase in the concentration of AgNO₃ decreased shoot production and shoot length. We also analyzed the effects of three different polyamines(putrescine,spermidine, and spermine) at various concentrations (10, 30, 70, and 100mg/L),in addition toMS with 2mg/L BAP, on shoot regeneration and length. Among these compounds, putrescine best augmented shoot regeneration and length, with the largest increase in the number of shoots (3.7/explant) and a moderate augmentation in shoot length 2.5cm at 70mg/LusingMS with 2mg/L BAP, followed by spermidine and spermine. This study revealed that the addition of ethylene inhibitors and polyamines couldimprove shoot regeneration and length in Aloe species. The procedures established in the present study could be used to produce a larger number of shoots, as well as enhance plant growth over a short period, and could be used as an important tool in future gene transfer studies.

KEYWORDS: Aloe saponaria; Meristem explants; Micropropagation;  Ethylene inhibitors; Polyamines

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