Effect of glyphosate on linear growth of seedling and thier anatomical characters of Hibiscus cannabinus Lin

Sanjay I. Kamble

Department of Botany and Coordinator Department of Seed Technology, Phulsing Naik Mahavidyalaya, Pusad Dist. Yavatmal India.

ABSTRACT: In the present investigation, effects of glyphosate on linear growth of seedlings have been studied. The seedlings have been reported to be most sensitive to the herbicidal action. Uniform seedlings were treated with different concentrations of herbicide for 24 hours. Then they were thoroughly washed with distilled water and kept for germination in petri dishes containing double layered of moistened filter paper at room temperature for 72 hours. The effect of herbicide on linear growth of hypocotyl and radicles was noted. Glyphosate was effective in checking the linear growth of seedlings. It inhibited the linear growth of seedlings and caused swellings. The radicle was found to be more susceptible than hypocotyl to this herbicide. The lethal dose was found to be 600 ppm for hypocotyl and 500 ppm for radicle. The anatomical changes like ruptured epidermis, disorganization of cortical cells in hypocotyl and radicle and disintegration of mesophyll cells were observed in cotyledon.

KEYWORDS: Herbicide; Glyphosate; Linear growth; Anatomical characters; Hibiscus cannabinus Linn

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