Effect of Borage on Hipocampal TNF-Α Protein And Gene  in The  Amyloid Β-Peptide(25–35)-Induced of Alzheimer Model in Rat

Ehsan Barati¹ Sara Soleimani Asl², Seyed Ali Pourbakhsh¹ , Mahmoud Jamshidian¹ and Siamak Shahidi²

¹Department of Microbiology,Science and Research Branch,Islamic Azad University,Tehran,Iran. ²Department of Neurophysiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan 65178-3-8736, Iran.

DOI : http://dx.doi.org/10.13005/bbra/2000

ABSTRACT: Alzheimer’s disease (AD) is a neuro degenerative disorder and most common form of dementia that leads to memory impairment. In the present study we have examined the investigating effect of Borago officinale on hipocampal TNF-α protein in the Beta Amyloid peptid induced of inflammation in the rat. Wistar male rats received intrahippocampal (IHP) injection of the Aβ(25–35) and borage extract orally (100 mg/kg).inflamation was confirmed by pathologist.in order to measure the expression of the TNF-α gene , RT- PCR method and for extraction of the TNF-α protein western bloting method was used. results showed that using Borage lead to reduce TNF-α gene and protein. This data suggests that borage could improve the reduction of inflammation factors such as TNF-α and borage consumption may lead to an improvement of AD-induced cognitive dysfunction.

KEYWORDS: TNF-α; Alzheimer; RT-PCR; Western Blot; Amyloid β-Peptide

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