Secretion and Characterisation of Ligninperoxidases by Some New Indigenous Lignolytic Fungi

J. K. Sharma², R. S. S. Yadav¹, N. P. Singh² and K. D. S. Yadav¹*

¹Department of Chemistry, D.D.U. Gorakhpur University, Gorakhpur - 273 009 India.

²Department of Chemistry, Udai Pratap College,Varansi - 221 002 India.

Corresponding Author E-mail: kds_chemistry@rediffmail.com

ABSTRACT: Secretion and enzymatic characteristics of ligninperoxidases from Coriolus versicolor MTCC 138, Trametes hirsuta MTCC 136, Phellinus linteus MTCC 1175, Volvariella volvacea MTCC 957, Phenerochaete chrysosporium MTCC 787, Pleurotus ostreatus MTCC 1803, Polyporous velutinus MTCC 1813, Daedalea elegans MTCC 1812, Pleurotus sajor caju MTCC 141, Pleurotus sapidus MTCC 1807 and Agaricus campestris MTCC 972 lignolytic fungi have been reported. Secretion of ligninperoxidase by these lignolytic fungi have been found to be in the range of 1.07 to 4.42 enzyme unit per ml of the culture medium. The enzymatic characteristics like Km, pH and temperature optima of ligninperoxidases from Coriolus versicolor MTCC 138, Trametes hirsuta MTCC 136, Phellinus linteus MTCC 1175, Volvariella volvacea MTCC 957, Phenerochaete chrysosporium MTCC 787, Pleurotus ostreatus MTCC 1803, Polyporous velutinus MTCC 1813, Daedalea elegans MTCC 1812, Pleurotus sajor caju MTCC 141, Pleurotus sapidus MTCC 1807 and Agaricus campestris MTCC 972 fungi have been determined using veratryl alcohol and H2O2 as the substrates. The values of Km using veratryl alcohol as the substrate have been found to be 61.0 μM, 65.0 μM, 59.0 M, 66.0 μM, 63.0 μM, 57.0 mM, 60.0 μM, 61.0 μM, 74.0 μM, 62.0 μM and 71.0 μM respectively. The values of Km using H2O2 as the substrate have been found to be 75.0 μM, 60.0 M, 65.0 μM, 70.0 μM, 90.0 μM, 75.0 μM, 80.0 μM, 80.0 μM, 75.0 μM, 60.0 μM, and 90.0 μM respectively. The pH optima values for ligninperoxidases of the above fungi have been found to be 2.4, 2.4, 2.5, 2.6, 2.6, 2.5, 2.5, 2.6, 2.5, 2.6 and 2.8 respectively, where as the temperature optima values were 25 °C, 25 °C, 24 °C , 25 °C, 25 °C, 25 °C, 26 °C, 25 °C, 25 °C, 25 °C and 25 °C respectively.

KEYWORDS: Lignin peroxidase; heme peroxidase; lignolytic enzyme

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