Antibacterial Protien (85kda) Produced by Lysinibacillus Sphaericus Against Methicillin Resistant Staphylococcus Aureus

V. Shanmugaraju¹*, N. Abirami², A. Mahalakshmipriya¹ and K. Selvam¹

¹Department of Boitechnology, Dr.NGP Arts and Science College, Coimbatore - 641 048 India. ²Department of Boichemistry, Dr.NGP Arts and Science College, Coimbatore - 641 035 India.   Corresponding Author E-mail: rajugenes@yahoo.co.in

ABSTRACT: Five marine isolates were isolated from marine water sample of seacoast Kollam Kerala ,India. The isolates were screened for antibacterial activity against Methicillin Resistant Staphylococcus aureus (MRSA) which is a major nasocomical pathogen.The results reveals that among five isolates one isolate(M5)showed the zone of inhibition of 24mm against MRSA .The cell free supernatant of M5 also showed the same activity and the proteinaceous nature of the compound for inhibition of MRSA growth was confirmed by enzyme treatment analysis and further 85KD of protein responsible for the observed activity was visualized while resolving the supernatant under SDS-PAGE. The isolate M5 was identified by 16srDNA sequencing and it reveals that isolate is Lysinibacillus sphaericus The 16srDNA sequence was aligned and phylogenetic relationship was studied.

KEYWORDS: Lysinibacillus sphaericus; antimicrobial peptides; MRSA; antibacterial protein

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