Optimization of Cultural Conditions for Cellulase Production by Aspergillus Niger (MTCC2196) Using Submerged Fermentation

R. Sharada¹, K. Swathi², S. Venkateshwar², M. Ananda Rao¹ and M. Narsi Reddy²

¹Department of Chemistry,University College of Science, Osmania University, Hyderabad India.

²Centre for Biotechnology,University College of Technology, Osmania University, Hyderabad India.

Corresponding Author E-mail: mnreddydr@gmail.com

ABSTRACT: Cellulases are complex enzymes and are capable of degrading lignocellulosic materials. These enzymes are produced by the fungus and bacteria. This work mainly focus on factors relevant for improvement of enzyme hydrolysis by using Aspergillus niger (MTCC2196) in submerged fermentation. Several cultural conditions were examined to access their effect in optimizing enzyme production. The Aspergillus niger (MTCC2196) could grow upto 28±2°C within a broad pH range of 3.0 to 6.0 with an optimal growth temperature and pH at 28°C and 5.0 respectively. For the Aspergillus niger (MTCC2196), cellulase production occurred between temperature 25°C to 30°C and pH 3.0 to 8.0. Maximum growth and enzyme production was observed at eighth day, when grown in 50ml of medium at pH 5.0, inoculum level 2ml and nitrogen sources like peptone 5mg, beef extract 5mg were added under shaking conditions at 280C. The results showed that Aspergillus niger (MTCC2196) under study is a good producer of cellulases.

KEYWORDS:

Cellulase; Aspergillus niger (MTCC2196); submerged fermentation; lignocellulosic material

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