Volume 6, number 2
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In Vitro Flowering and Regeneration of Centella Asiatica

N. Sarada Mani1, Rohini Kiran Kunta1, 2 and Varahala rao Vadlapudi1,2

1Department of Botany, Andhra University, Vishakhapatnam (India). 2Dr. Lankapalli Bullyya Post Graduate College, Visakhapatnam (India).

ABSTRACT: In vitro plant regeneration was achieved from explants of nodal segments on MS medium containing different concentrations and combinations of auxins and cytokinins. In vitro flowering was noticed from nodal explants cultured on MS medium supplemented with BAP 2 mg/L + NAA 1mg/L. Multiple shoot induction was observed on MS medium supplemented with BAP + NAA (0.5 - 4 mg/L). The regenerated shoots were transferred onto MS Medium fortified with IBA for root induction. The regenerated plantlets have been successfully established in soil and they were shifted to field condition.

KEYWORDS:

Plant regeneration; auxins; Multiple shoot induction; MS Medium

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Mani. N. S, Kunta. R. K, Vadlapudi. V. R. In Vitro Flowering and Regeneration of Centella Asiatica. Biosci Biotechnol Res Asia 2009;6(2)
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Mani. N. S, Kunta. R. K, Vadlapudi. V. R. In Vitro Flowering and Regeneration of Centella Asiatica. Biosci Biotechnol Res Asia 2009;6(2). Available from: https://www.biotech-asia.org/?p=9000.

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