Evaluation and Concentration of Polyethylene Glycol Assay for Early Diagnosis of Dengue Antigen 2

K. Jaianand*, P. Gunasekaran And A. K. Sheriff

Department of Virology, King Institute of Preventive Medicine, Chennai - 600 032 India.

Corresponding Author E-mail:jaianand.k143@gmail.com

ABSTRACT: Dengue, a mosquito borne viral infection in recent years is been a major global public health problem with increasing number of reports of Dengue fever and Dengue hemorrhagic fever with usual manifestations every year. In spite of availability of various diagnostic methods including high sensitive molecular techniques, WHO recommends, ‘Haemagglutination-inhibition (HAI)’ over expensiveness and non-feasibility of others. Therefore there is a need for time saving and in expensive method for concentration. This report conveys that polyethylene glycol (PEG) for concentration of arboviruses had been used for rapid diagnosis of Dengue type II viruses. In this study two methods using PEG were evaluated for concentration and are recommended as an initial step in the concentration of arboviruses propagated in tissue culture, as it is simple, rapid, and inexpensive.

KEYWORDS: Polyethylene glycol (PEG); Haemagglutination-inhibition (HAI); Dengue type II viruses

[ HTML Full Text]