Volume 7, number 1
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Optimization of Process Variables for the Microbial Degradation of Phenol by Pseudomonas Fluorescence (NCIM 2100)

V. Sridevi1, M. V. V. Chandana Lakshmi1, A. V. N. Swamy2, M. Narasimha Rao3 and S. V. Naidu3

1Centre for Biotechnology, Department of Chemical Engineering, Andhra University, Visakhapatnam - 03 India. 2Department of Chemical Engineering, College of Engineering, J.N.T.U., Anantapur India. 3Al-Ameer College of Engineering and Information Technology, College of Engineering, Gudilova, Anadapuram, Visakhapatnam India.   Corresponding Author E-mail:vellurusridevi@yahoo.co.in

ABSTRACT: Phenol biodegradation by Pseudomonas fluorescence (NCIM 2100) was performed in batch system. Experiments were carried out as a function of inoculum size (1-10 % v/v), p H (5-9) and temperature (27-32°C). Optimization of these three process parameters for phenol biodegradation was studied. Optimum inoculum size, p H and temperature were determined as 5% v/v, 7 and 30°C respectively. At these optimum conditions, the degradation capability of P. fluorescence was carried out at phenol levels (100- 750 mg/l). The maximum phenol degradation of P. fluorescence was 480 mg/l.

KEYWORDS: Biodegradation; phenol; Pseudomonas fluorescence; inoculum size; pH; temperature.

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Sridevi V, Lakshmi M. V. V. C, Swamy A. V. N, Rao M. N, Naidu S. V. Optimization of Process Variables for the Microbial Degradation of Phenol by Pseudomonas Fluorescence (NCIM 2100). Biosci Biotech Res Asia 2010;7(1)
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Sridevi V, Lakshmi M. V. V. C, Swamy A. V. N, Rao M. N, Naidu S. V. Optimization of Process Variables for the Microbial Degradation of Phenol by Pseudomonas Fluorescence (NCIM 2100). Biosci Biotech Res Asia 2010;7(1). Available from: https://www.biotech-asia.org/?p=9574

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