Indirect Somatic Embryogenesis in Emblica : A Preliminary Study in our Research Lab

Preeti Madharia and Amit Kumar Dutta

Department of Biotechnology, Rungta College of Science and Technology (Affiliated Pt. Ravishankar Shukla University, Raipur), Durg (CG). India.

ABSTRACT: Emblica, common name Amla, are widely used in the Indian system of medicine and believed to increase defense against diseases. Different activities of Amla are today well known and famous for all persons as well as for all researchers. Seeds and different seedling parts were used as explants. These explants were surface sterilized and Seedling parts were induced on Murashige & Skoog's (MS) liquid medium with hormone. The seedling part of Emblica was further subculture on MS with different hormone concentration. The in vitro generated callus with shoot initiation was rooted on rooting media with different concentration of hormone. By this method 60% shoots were rooted. Only 46% shoots were rooted within 2-3 weeks in this method. The in vitro rooted plants could be acclimatized and hardened in green house and transplanted to the field successfully. This experiment will help in the large scale plant production of Emblica. Further long term study is required to get final conclusion.

KEYWORDS: Emblica; Diseases; Indian System of Medicine

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