Volume 7, number 1
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Extractive Spectrophotometric Determination of Tenofovir

K. Vanitha Prakash1, M. Padmalatha2 and Eranna Dopadally3

1SSJ College of Pharmacy, V.N. Pally, Gandipet, Hyderabad India.

2Vijaya College of Pharmacy, Munganoor, Hayathnagar, Hyderabad India.

3Sultan-Ul-Uloom College of Pharmacy, Banjara Hills, Hyderabad India.

Corresponding Author E-mail:prakash.karnam@gmail.com

ABSTRACT: Two simple, economical, precise, reliable and reproducible visible spectrophotometric methods (A and B) have been developed for the estimation of Tenofovir (TNF) in bulk as well as in Tablet formulations. The developed methods A and B are based on the formation of chloroform extractable complex of Tenofovir with Bromocresol green (Method A) and Erichrome (Method B), which shows absorbance maxima at 420 nm and 510 nm respectively. The absorbance-concentration plot is linear over the range of 25-250 mcg/ml for Method A, and 25-200 mcg/ml for Method B. The different experimental parameters affecting the development and stability were studied carefully and optimized. Results of analysis for all the methods were validated statistically and by recovery studies.

KEYWORDS: Tenofovir; Bromocresol green; Erichrome; Ultraviolet-Visible double beam spectrophotometer.

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Prakash K. V, Padmalatha M, Dopadally E, Extractive Spectrophotometric Determination of Tenofovir. Biosci Biotech Res Asia 2010;7(1)
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Prakash K. V, Padmalatha M, Dopadally E, Extractive Spectrophotometric Determination of Tenofovir. Biosci Biotech Res Asia 2010;7(1). Available from: https://www.biotech-asia.org/?p=9740

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