Volume 7, number 1
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Isolation and Characterization of Thermophilic Bacteria from Hot Spring n Orissa, India

H. K. Khuntia, S. Samal, R.K. Sahoo, S. K. Kar, B. B.Pal* and K. Mala Bihari

Microbiology Division, Regional Medical Research Centre, Chandrasekharpur, Bhubaneswar- 751 023 India. Corresponding  Author E-mail:bbpal_rmrc @ yahoo.co.in

ABSTRACT: The present study was conducted to isolate thermophilic bacteria from hot spring at Tarabalu Orissa, India and to study its physiological and biochemical properties The strain from hot springs at Tarabola was isolated by plating and screening on Nutrient Agar medium. It was characterized by the physiological, biochemical and antimicrobial analysis. The cells were Gram-negative, motile rods, non-spore forming and generally occurred singly or in pairs. The growth temperature ranges from 570 C to 1000 C, optimum at 750 C; growth pH ranges from 5.5 to 9.0, optimum at 7.0. The strains used glucose and lactose as carbon and energy sources. The hot spring isolate was active for enzyme Amylase at 570 C. Further molecular study is required for its identification and taxonomic characterization.

KEYWORDS:

Thermophillic, hot spring, bacteria, enzyme, temperature.

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Khuntia H. K, Samal S, Sahoo R. K, Kar S. K, Pal B. B, Bihari K. M, Isolation and Characterization of Thermophilic Bacteria from Hot Spring n Orissa, India. Biosci Biotech Res Asia 2010;7(1)
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Khuntia H. K, Samal S, Sahoo R. K, Kar S. K, Pal B. B, Bihari K. M, Isolation and Characterization of Thermophilic Bacteria from Hot Spring n Orissa, India. Biosci Biotech Res Asia 2010;7(1). Available from: https://www.biotech-asia.org/?p=9746

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