Volume 8, number 2
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Lectin from the Seeds of Wild Bitter Gourd (Momardica Charantia L. Var. Jhalri): Isolation and Partial Charactrization

Ajay Pratap Singh* and K. D. Sexena

Department of Chemistry, Bareilly College, Bareilly - 243 001 India

Correspondin Author E-mail: ajayojas@ yahoo.co.in

ABSTRACT: A Lectin was isolated from the seeds of Momardica charantia L.var. Jhalri (MCJ) using ammonium sulphate fractionation at pH 6.8 and 4°C. The purification of isolated lectin was done by ion exchange chromatography using DEAE-Cellulose with discontinuous gradient of acetate buffers. Purity and homogeneity of MCJ lectin was confirmed by 10% SDS-PAGE at pH 8.6. The same technique revealed its apparent molecular weight of 128kD indicating it to be a high mol. wt. lectin among the members of Cucurbitaceae family. MCJ lectin showed potent heamagglutination activity for O+ erythrocytes. The total lectin isolated was 118.80 mg per 100 g seeds. Climatic and ecological factors under which the plant grows up were assumed to be responsible for high yield of MCJ lectin.

KEYWORDS: Lectin; SDS-PAGE; Haemagglutination; Cucurbitaceae

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Singh A. P, Sexena K. D. Lectin from the Seeds of Wild Bitter Gourd (Momardica Charantia L. Var. Jhalri): Isolation and Partial Charactrization. Biosci Biotech Res Asia 2011;8(2)
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Singh A. P, Sexena K. D. Lectin from the Seeds of Wild Bitter Gourd (Momardica Charantia L. Var. Jhalri): Isolation and Partial Charactrization. Biosci Biotech Res Asia 2011;8(2). Available from: https://www.biotech-asia.org/?p=9829

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