Evaluation of antibacterial activities of aqueous and methanolic extracts of Juglans regia bark (Derum) against some opportunistic oral bacteria

Asmaa A. Faden

Department of Oral Medicine and Diagnostic Sciences, College of Dentistry, King Saud University, Saudi Arabia, Riyadh- 11545, P.O. Box 60169

Corresponding Author E-mail: afaden@ksu.edu.sa

DOI : http://dx.doi.org/http://dx.doi.org/10.13005/bbra/1048

ABSTRACT:

The antibacterial properties of the aqueous and methanolic extracts of Juglans regia bark known as (Derum: a traditional wooden stick used as an oral cleanser and lip dye, especially among women in some areas in Saudi Arabia) were studied against some bacteria capable of causing opportunistic infections in the mouth. The objective of this study is to investigate the antibacterial activities of aqueous and methanolic extracts of Juglans regia against Escherichia coli and Staphylococcus aureus. Juglans regia studies showed antibacterial activity which varied depending on the Juglans regia extracts method and concentration and the bacterium under test. Five percent of the aqueous and methanolic extracts were added to growth medium of Escherichia coli or Staphylococcus aureus. The extract that caused growth inhibition (aqueous) was purified using membrane filter (0.45µm). Antibacterial activities of the obtained fractions were assessed using agar-well diffusion test. Five percent aqueous extract inhibited the growth of Escherichia coli or Staphylococcus aureus, while methanolic extract was ineffective. In conclusion aqueous extract of the Juglans regia bark “Derum” contains a potent antibacterial agent that is protein in nature and may be useful in the treatment of some oral infections.

KEYWORDS: Juglans regia;Staphylococcus aureus'; Escherichia coli; MIC; MBC; Derum

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