Volume 9, number 2

Strong Inhibition of Jack Bean Urease by Chromium (III)

G. Rezaei Behbehani1, L. Barzegar2 and M. Mohebbian3

1Department of Chemistry, Faculty of Science, Islamic Azad University, Takestan Branch, Takestan, Iran. 2Department of Chemistry, Payame Noor University (PNU), Abhar, Iran. 3Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. 4Department of Biological Sciences, Tarbiat Moallem University, Tehran, Iran.

DOI : http://dx.doi.org/http://dx.doi.org/10.13005/bbra/1053

ABSTRACT:

Urease activity is often used for characterization of microbial viability in soil. The aim of the investigations was to measure the influence of chromium (III) on urease activity. Urease activity in pure solution was so sensitive for Cr3+, which caused inhibition of urease activity significantly. In this paper complexation reaction between Cr3+ and Jack bean urease is examined using isothermal titration calorimetry (ITC). It was found that chromium ions acted as a noncooperative inhibitor of JBU, and there is a set of 12 identical and independent binding sites for Cr3+ ions. The association equilibrium constant is 6.79-106 L-1.mol, indicating the strong interaction of Cr3+ ion with JBU. The molar enthalpy of binding is DH = 15.10 kJmol-1.

KEYWORDS: Isothermal Titration Calorimetry, Jack bean urease, Cr3+ ion, binding parameters.

Copy the following to cite this article:

Behbehani G. R, Barzegar L, Mohebbian M. Strong Inhibition of Jack Bean Urease by Chromium (III). Biosci Biotech Res Asia 2012;9(2)
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Behbehani G. R, Barzegar L, Mohebbian M. Strong Inhibition of Jack Bean Urease by Chromium (III). Biosci Biotech Res Asia 2012;9(2). Available from: https://www.biotech-asia.org/?p=10043

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