In vivo Animal Model for Screening of Anti Diabetic Activity

S. Raju and K. Hemamalini*

Teegala Ram Reddy College of Pharmacy, Meerpet, Hyderabad - 500 097, India.

DOI : http://dx.doi.org/http://dx.doi.org/10.13005/bbra/1062

ABSTRACT:  

he Antidiabetic activity of Kigelia africana (Lam.) (Family: Bignoniaceae) and Tabebuia rosea(Bertol) DC (Family: Bignoniaceae) were investigated in Alloxan induced diabetic albino rats. A comparison was made between both the plant extracts and a known anti diabetic drug Glibenclamide (5 mg/kg body weight). The dried leaves of Kigelia africana and Tabebuia rosea were subjected to extraction by continuous hot percolation using methanol as solvent and were subjected to standardization using pharmacognostical and Phytochemical screening. Dose selection was made on the basis of acute oral toxicity study (200 mg/kg body weight) as per OECD and CPCSEA guidelines. Oral administration of extracts of Kigelia africana (200mg/kg) and Tabebuia rosea (200mg/kg) for 7 days resulted in a significant reduction in blood glucose levels. Alloxan induced diabetic rat model was used for the evaluation of anti diabetic activity. Activity is more for Tabebuia rosea in comparison with Kigelia africana. Methanolic extract of Tabebuia rosea and Kigelia africana showed significant (p<0.001) anti diabetic activity. These extracts also prevented body weight loss in diabetic rats. The drug has the potential to act as an anti diabetic drug.

KEYWORDS: Kigelia africana; Tabebuia rosea; antidiabetic activity; Alloxan;Acute oral toxicity.

[ HTML Full Text]