Cefixime Trihydrate (CEF), Ornidazole (ORD), RP-HPLC, Validation.

A. Ayesha¹, M. N. Abubacker²*, G. S. Rampradheep¹ and M. Sivaraja³

¹Department of Civil Engineering, Kongu Engineering College, Perundurai, Erode, TN, India. ²Department of Biotechnology, National College, Tiruchirappalli - 620 001, India. ³Principal, NSN College of Engineering & Technology, Karur, India.

DOI : http://dx.doi.org/ http://dx.doi.org/10.13005/bbra/1065

ABSTRACT: Concrete is found to be an essential building material. Though concrete is quite strong mechanically, it suffers from several drawbacks, such as low tensile strength, permeability to liquids, corrosion of reinforcement, susceptibility to chemical attack. Consequently concrete develops cracks affecting the durability of the structure. Modifications have been made from time to time to overcome such drawbacks of concrete all of which are not easy and result oriented. Therefore bacterial concrete technique is developed using some bacterial species like Bacillus subtilis, B. pasteruii and Pseudomonas aeruginosa. The aim of this study is to use B. subtilis bacterial culture to produce microbial concrete. Bacterial concrete is a novel technique in which the bacterial enzymes induce carbonate precipitation resulting from metabolic activities of B. subtilis in concrete to improve the overall behaviour of concrete. The microbial induced calcium carbonate precipitation (MICCP) involves a series of biochemical reactions in which the microbe B. subtilis produces urease, which catalyzes urea to produce CO2 and ammonia, resulting in an increase of pH in the microbial concrete during which the ions Ca2+ and Co2–3 precipitate out as calcium carbonate in the form of calcite (CaCO3) which in turn has crack healing properties. This study is focused on the compressive strength, water absorption rate, split tensile strength of cement mortar (bacterial concrete), urease production capacity of B. subtilis bacteria as well as the evaluation of calcite mineral producing capacity by standard methodology. The development of the bacterial concrete using B. subtilis will provide the basis for an alternative and high quality concrete which will be environmentally safe, improve the resistance to cracks and enhance the durability of building materials induce carbonate precipitation resulting from metabolic activities of B. subtilis in concrete to improve the overall behaviour of concrete in compressive strength of cement mortar. The bacterial induced calcium carbonate precipitation (MICCP) involves a series of biochemical reactions in which B. subtilis produce urease, which catalyzes urea to produce CO2­ and ammonia, resulting in an increase of pH in the bio-cement where ions Ca+ and CO3 precipitate calcium carbonate in the form of Calcite (CaCO3) which in turn has crack healing properties.

KEYWORDS: Baterial Concrete; Bacillus subtilis; Carbonate precipitation;crack healing properties.

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