Volume 9, number 2

Estimation of the Immunocompetance Status of Kadaknath

D.P. Saxena1, R. Stephan2, S.K. Mishra4, S. Shukla3, R. Saxena1, S.O. Pratap4 and D.P. Singh4

1Vinayaka Mission University, Salem, T.N., India. 2Govt. Arts College, Department of Botany, Ariyaloor, T.N., India. 3Integral University, Lucknow, U.P., India. 4Central Avian Research Institute, Izatnagar, Bareilly, U.P., India. Corresponding Author E-amil: saxenadps1234@gmail.com

DOI : http://dx.doi.org/http://dx.doi.org/10.13005/bbra/1067

ABSTRACT:

ABSTRACT

The native chicken were studied separately male and female line by checking immunocompetence through injected by SRBC with intravenous injection for the low and high antibody response through the 5th day post immunization. Divergence between the high and low lines selected for SRBC antibody was immediate and increased during selection. The chicken line of kadaknath breed immunized by SRBC for humoral immune response by the some reagent such as Alsever’s Solution and PBS used and after these processes collected of blood under preserved condition In the presence of anticoagulant. We investigated of heamaglutination test by using micro titer plate and determined IgG also against SRBC. Humoral response by sheep RBC and agglutination test. Antibody titers against ND were determined by HI assay using 4 HA units of NDV. The Kadaknath population included either sex wise both male and female, were analyzed for immunocompetence traits. Including the both sex, HA results showed that antibodies titter against SRBCs was recorded with overall mean of 7.69 ±0.14 for purebred Kadaknath chickens and for the identification of IgG through the analysis of these parameter is affected the same effect for the both male and female and those were recorded overall mean is 1.87 ±1.08. In the analysis (SPSS software) of both male and female kadaknath chicken is standard error of mean is 0.137 ±0.063 same for humoral response against SRBC. From this investigation of kadaknath chicken using the mean absorbance value for each sample, determine the corresponding concentration of anti-SRBC IgG in u/ml from the standard curve.

KEYWORDS: Chicken; Immuneresponce; IgG; Heamaglutination titer; In-ovo vaccination.

Copy the following to cite this article:

Saxena D. P, Stephan R, Mishra S. K, Shukla S, Saxena R, Pratap S. O, Singh D. P. Estimation of the Immunocompetance Status of Kadaknath. Biosci Biotech Res Asia 2012;9(2)
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Saxena D. P, Stephan R, Mishra S. K, Shukla S, Saxena R, Pratap S. O, Singh D. P. Estimation of the Immunocompetance Status of Kadaknath. Biosci Biotech Res Asia 2012;9(2). Available from: https://www.biotech-asia.org/?p=10134

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