Protein Analysis of Dengue Fever Vector Aedes Aegypti, Using SDS-PAGE in Jeddah Governorate-Saudi Arabia

Abbas M. Al-Azab¹, Khalid M. Al-Ghamdi^2*, Mohamed A. Shaheen³ and Ahmed A. Zaituon⁴

^1,3,4Department of Arid land Agriculture, Collage of Meteorology, Environment and Arid land Agriculture, ²Department of Biological Sciences - King Abdulaziz University, Saudi Arabia.

DOI : http://dx.doi.org/10.13005/bbra/1097

ABSTRACT: Protein samples of (larva, pupa and adult) for lab and field strains of dengue fever vector, Ae. aegypti were isolated. Proteins were analyzed using sodium dodecyl sulfate polyacrylamide (SDS-PAGE).The SDS-PAGE analysis showed that, total of 6 protein bands were detected. The relative molecular weight of the detected bands was approximately in the range of 16.6 – 75.6 kDa. The 16.6 kDa protein band was observed through all insect stages. In contrary, protein bands corresponding to (19.1 ,41.7 and 75.6 kDa) were identified specifically in adults of field strain whereas, the lab strain adults revealed specific protein band of 36 kDa. In the meantime, pupa protein pattern exhibiting four protein bands with molecular weight of 16.6, 46.8 , 69 and 75.6kDa The obtained results provide basic information would be utilized for the identification of differentially specific protein as a target oriented for specific agent of Ae. aegypti. Further studies are needed to identify these protein and their biological roles in dengue fever infections to be implemented in integrated insect pest management.

KEYWORDS: Dengue fever; Aedes aegypti; protein profiles; SDS-PAGE

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