Isolation and Characterization of Indole Acetic Acid (IAA) Produced by a Halo Tolerant Marine Bacterium Isolated from Coastal Sand Dune Plants

M. Jayaprakashvel¹, K. Abishamala², C. Mathan Periasamy², J Satheesh³, A. Jaffar Hussain³ and  M.C.Vanitha^3*

¹Department of Marine Biotechnology, AMET University, Kanathur, Chennai - 603112, India ²Department of Biotechnology, Sree Sastha Institute of Engineering and Technology, Kattankulathur - 603203, India ³Tamil Nadu Pollution Control Board, Palladam, Coimbatore- 641664, India ⁴Centre for Marine Bioprospecting AMET University, Kanathur, Chennai-603112, India

DOI : http://dx.doi.org/10.13005/bbra/1419

ABSTRACT: Halotolerant bacteria are capable of tolerating the presence of saline stress and can even survive in the absence of saline stress. This study was done to enhance Indole Acetic Acid production by media optimization and then to isolate the same.. The work starts with sub culturing of the 46 halotolerant rhizobacterial (HTRB) strains from the culture collection of previously isolated bacteria. These HTRB strains were screened for the production of Indole Acetic Acid and further quantified with UV-VIS spectrophotometer. It has been found that strain AMET7041 has produced higher amount of IAA and hence was selected for further studies. Media parameters such as Carbon, Nitrogen, Tryptone and Salinity were altered for enhanced IAA production. The optimized parameters were used to mass culture strain AMET7041. Further, the IAA responsible for the plant growth promotion was also isolated, separated and authenticated via standard methodologies. The culture filtrate was concentrated and then components separated using silica gel column chromatography. The IAA was separated using various concentrations of solvents Hexane and Chloroform in silica gel column chromatography and its identity was confirmed in TLC as well as UV Visible spectroscopy.

KEYWORDS: Halotolerant Rhizobacteria; IAA; plant growth; purification; Column Chromatography

[ HTML Full Text]