Volume 13, number 2
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Screening of Mutation in Parkin Gene – Exon 3 for Diagnosis of Parkinson’s Disease

R. Thirugnanasambandam1*, A. M. Sabitha Rani2, L. Stanley Abraham1 and Vasantharaja Raguraman1

1Centre for Ocean Research, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai – 600119.

2Department of Biotechnology, Prince Shri Venkateshwara Arts and Science College, Gowrivakkam, Chennai –600091.

ABSTRACT: The present study focuses to screen the mutation in parkin gene (exon3) of Parkinson’s diseased patients by collecting blood samples from 16 early onset Parkinson’s disease patients in the age group of below 45 years. To detect these mutations, we performed an effective technique based on the real-time TaqMan PCR system. The amplified product was subjected to sequence analysis for confirming mutation in Parkin gene (exon3). The chromatogram was collected and subjected to sequence alignment using BLAST software. The sequenced exon 3 was visualized for the presence of Mutation. In this study, we have not identified any mutation in exon 3 and conclude that there are possibilities for the involvement of other exons in induction of this disorder to become the basis for a diagnostic test.

KEYWORDS: Parkinson’s disease; DNA; gene; parkin; exon 3; mutation

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Thirugnanasambandam R, Rani A. M. S, Abraham L. S, Raguraman V. Screening of Mutation in Parkin Gene – Exon 3 for Diagnosis of Parkinson’s Disease.
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Thirugnanasambandam R, Rani A. M. S, Abraham L. S, Raguraman V.Screening of Mutation in Parkin Gene – Exon 3 for Diagnosis of Parkinson’s Disease. Biosci Biotech Res Asia 2016;13(2). Available from: https://www.biotech-asia.org/?p=12592

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