Effect of Metabotropic Glutamate Receptors II /III Agonists on Spike-Wave Discharge in Primary Somatosensory Perioral Cortex of Male WAG/Rij Rats

Abotaleb Ghorbanzade¹, Parviz Shahabi^2*, Firouz Gaderi Pakdel³, Hadi Ebrahimi⁴, Mina Sadighi⁵, Behnaz Sadeghzadeh Oskouei¹ and Hadi Yousefi⁴

¹Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

²Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

³Neurophysiology Research Center, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.

⁴Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

⁵Department of Physiology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.

DOI : http://dx.doi.org/10.13005/bbra/2174

ABSTRACT: The aim of this study was investigation of metabotropic glutamate receptors II/ III on spike-and-wave discharges in perioral somatosensory cortex of male WAG/Rij rats. Eighteen male WAG/Rij rats was used in this experiment. Rats anesthetized with a combination of ketamine and xilusinip and 2 μl of drugs injected with a 10 μl Hamilton syringe bilaterally. At the end of the experiment, all animals were killed by high dose of ketamine. The brain was removed rapidly and fixed in 10% formalin. Cresyl violet stained coronal sections were viewed with a light microscope to verify cannula placement. In all groups mean numbers and mean duration of Spike-and-wave discharges was decreased, but peak frequency was only in L-AP4 group was reduced. These agonists are able to suppress epileptic discharges in WAG/Rij rats and could be useful for treatment of Absence epilepsy in the future.

KEYWORDS: Metabotropic; Glutamate; WAG/Rij; Spike-and-wave; Perioral somatosensory cortex

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