The Influence of Lecithin Administration on Hepatic Expression FMO3 and FMO5 Genes in N-Mary Mice

Abbas Mohammadi^1,2, Mohammad Reza Baneshi³, Zakaria Vahabzadeh⁴ and Tahereh Khalili^1,*

¹Department of Clinical Biochemistry,Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.

²Physiology Research Center, Kerman University of  Medical Sciences, Kerman, Iran.

³Modeling in Health Research Center, Institute for Futures Studies in Health, Department of Biostatistics and Epidemiology, School of Public Health, Kerman University of Medical Sciences, Kerman, Iran.

⁴Clinical biochemistry department, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.

Corresponding Author E-mail: taherehkh2131@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2332

ABSTRACT: To investigate the effect of lecithin on FMO3 and FMO5 gene expression, L-α-lecithin or ethanol 30% (as control) was administrated to female N-mary mice as intraperitoneal injection and animals were sacrificed 48h after treatment. The relative expression of mRNA and protein were assessed by real-time PCR and western blotting analysis. In this study, FMO5 expression was also evaluated in HepG2 cell line. FMO3 mRNA expression in mice liver was increased to 1.8-fold in animal received lecithin compared to control group, While FMO5 expression did not showed any changes as in vivo and in vitro experiments. The our results are suggested to induce of FMO3 expression by lecithin.

KEYWORDS: Lecithin; flavin-containing monooxyganase; gene expression

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