Immunomodulatory and Macrophage Activating Activity of Lactobacillus fermentum DLBSA204 in Response to Respiratory Infection in a Cellular Model

Anggi Shestita Wulandari, Olivia Mayasari Tandrasasmita, and Raymond Rubianto Tjandrawinata

Dexa Laboratories of Biomolecular Sciences, PT Dexa Medica, Industri Selatan V, Block PP No. 7, Jababeka Industrial Estate II, Cikarang, West Java, 17550, Indonesia.

Corresponding Author E-mail: raymond@dexa-medica.com

DOI : http://dx.doi.org/10.13005/bbra/2269

ABSTRACT: Lactobacillus fermentum DLBSA204 is an isolated lactic acid bacterium (LAB) from human breast milk. This study focused on the effects of L. fermentum DLBSA204 on macrophage cell proliferation with MTS assay using murine RAW 264.7 macrophage-like cell line as well as related molecular changes associated with macrophage activation. It was demonstrated that L. fermentum DLBSA204 can activate macrophage cell by increasing Nitric Oxide and cytokine expression level. Moreover, this LAB was able to increase phagocytic activity of macrophage. L. fermentum DLBSA204 was also able to inhibit the adhesion of Streptococcus pneumoniae on lung cells. In conclusion, L. fermentum DLBSA204 works as an immunomodulator and macrophage activator.

KEYWORDS: Lactobacillus fermentum; immunomodulator; macrophage; Streptococcus pneumoniae

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