Molecular Diagnosis on Glucose 6 Phosphate Dehydrogenase in a Sample of Iraqi Patients

Rehab S. Ramadhan¹, Hadeel M. Khalaf¹ and Rebah N. Jabber²

¹Department of Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq.

²Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq.

Corresponding Author E-mail: rehabrebah2004@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2354

ABSTRACT: This study was constructed to invertigate  Glucose 6 phosphate dehydrogenase deficiency, and the genetic disorder that leading to heamolysis anemia in group of Iraqi patient. A total of 50 blood samples were collected from different hospitals (Yarmook Hospital, Child hospital , AL Alweyaa , and Medical City) these samples varied in severity in of the enzyme deficiency from mild to chronic according to the test of G6PD, and 20 samples served as control (healthy). The period time of samples collection took about three months from March to June 2014. The percentage of mild symptoms was 42% , acute symptoms was 40% and carrier G6PD was 18% as stated on statistics analysis of Chi square. DNA was extracted from samples and subjected to PCR amplification using 3 specific primers designed for purpose of this study, the first primer (Frag I) with product length 115 bp, second primer (FragII) with product length 1500 bp, third primer (Frag III) with product length 2000 bp that amplify the specific site for the carrier and mild patients with G6PD ,while no band was amplified for the chronic patients with G6PD. After comparing cases and with NCBI we found that the percentage insertion was 30% and substituted was 70%.

KEYWORDS: G6PD; type mutation of gene; heamolysis anemia; and molecular diagnosis

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