Immunomodulatory Effects of Helicobacter Pylori on Pro and Anti-Inflammatory Cytokines Production in Peripheral Whole Blood Cells Culture

Nazila Bahmaie¹, Soghrat Faghihzadeh², Abdolreza Esmaeilzadeh^*3 and Bahram Amini⁴

¹Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of medical sciences, Zanjan, Iran.

²Department of Biostatistics and Epidemiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan,Iran.

³Department of Immunology and Cancer Gene Therapy Research Center, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

⁴Department of Microbiology and Virology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.

Corresponding Author E-mail: a46reza@zums.ac.ir

DOI : http://dx.doi.org/10.13005/bbra/2387

ABSTRACT: Recent documentations about sepsis as a systemic inflammatory response and inefficiency of some antimicrobial treatments in immunological disordersoutline that any intervention modifying the immune response with therapeutic intent, will be rational. Helicobacter Pylori (H.pylori), with major role in etiopathogenesis of duodenum peptic ulcer and gastric lymphoma, exerts itʼs immunomodulatory properties with long time intestinal colonization and protection against several certain inflammatory disorders. Due to theunclearness ofthis pathogenimmunomodulatory effects on whole blood cells culture, the aim of this study is evaluation of H.pylori whole body immunomodulatory effectson whole blood cells culture. In this study, after H.pyloriisolation from a pathological positive biopsy specimen, it was cultured in Brucella Blood Agar. Then, bacteria was co-cultured with whole blood cells in RPMI-1640 for 24h with CO₂ incubation. At last, TNF-alpha and TGF-beta concentrations of supernatants were assayed by quantative ELISA. Finally, non-parametric tests were measured out for data analysis. Our results showed upregulation of TGF-betaand TNF-alphasecretion, exposure toH.pylori (p value 0.017 and 0.018, respectively) compared to their untreated culture. These data show that immune response of H.pylori maypromote pro and anti-inflammatory responses. It seems that clinical applications of cytokines secretion, may be a promising window for further investigation leading to improvement of immune related diseases.

KEYWORDS: Immunomodulation; Helicobacter pylori; Whole blood cells; Cytokine

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